Product description
Glycogen phosphorylase (Glycogen phosphorylase, GP, EC 2.4.1.1) is a key enzyme in glycogen metabolism, breaking α-1,4-glycosidic bonds from the non-reducing end of glycogen molecules to remove glucose residues and release glucose-1-phosphate until it reaches four glucose residues before the α-1,6-glycosidic bond branching point of the glycogen molecule. GP is divided into active glycogen phosphorylase a (GPa) and inactive glycogen phosphorylase b (GPb). GPb can be activated in the presence of a certain concentration of adenosine monophosphate (5'-AMP). This kit provides a rapid, sensitive, and simple detection method. GP catalyzes glycogen and inorganic phosphorus to produce glucose residues, forming glycogen and glucose-1-phosphate. Phosphoglucomutase and glucose-6-phosphate dehydrogenase then catalyze the reduction of NADP+ to NADPH, which reacts with a specific color reagent to form a colored substance. By detecting the increase in the colored substance at 450nm, the GP enzyme activity can be calculated. When a certain concentration of adenosine monophosphate (5'-AMP) is added, the activity of GP (GPa and GPb) is measured. When adenosine monophosphate (5'-AMP) is not added, the activity of GPa is measured. The activity of GPb is obtained by subtracting the activity of GPa from the activity of GP.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice.