Product description
Glycogen phosphorylase (Glycogen phosphorylase, GP, EC 2.4.1.1) is a key enzyme in glycogen metabolism, breaking α-1,4-glycosidic bonds from the non-reducing end of glycogen molecules to remove glucose residues and release glucose-1-phosphate, until it reaches four glucose residues before the α-1,6-glycosidic bond branching point of the glycogen molecule. GP is divided into active glycogen phosphorylase a (GPa) and inactive glycogen phosphorylase b (GPb). Glycogen degradation mainly occurs under the catalysis of GPa. This kit provides a rapid, sensitive, and simple detection method. GPa catalyzes glycogen and inorganic phosphorus to produce glucose residues, forming glycogen and glucose-1-phosphate. Phosphoglucomutase and glucose-6-phosphate dehydrogenase then catalyze the reduction of NADP+ to NADPH, which reacts with a specific color reagent to form a colored substance. By detecting the increase in the colored substance at 450nm, the GPa enzyme activity can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), water bath, desktop centrifuge, pipette, mortar and pestle, ice.