Product description
Glycogen is a high molecular weight substance formed by the polymerization of glucose molecules through glycosidic bonds, stored as an important energy substance in vital organs such as the liver, muscles, and brain. Abnormalities in the storage or metabolism of glycogen can cause a variety of diseases; therefore, measuring changes in glycogen content is of great significance for studying glycogen metabolism and related diseases. The anthrone method is used: a strong alkaline extraction liquid is used to extract glycogen, and concentrated sulfuric acid dehydrates glycogen to produce sugar aldehyde derivatives, which react with anthrone and have a maximum absorption peak at 620nm. The glycogen content is then quantified by comparing it with a glucose standard solution treated with the same method
Additional Materials and Equipments Required
Microplate reader, water bath, pipette, 96-well plate, concentrated sulfuric acid (not allowed for courier), and distilled water.