Product description
Glutathione transferase (GST; EC 2.5.1.18) is a family of proteins with multiple physiological functions, clearing potentially toxic compounds, including those produced by oxidative stress, and is part of the cellular defense mechanism. GST catalyzes the combination of GSH with CDNB, and the combined product has an absorption peak wavelength of 340nm; by measuring the rate of increase in absorbance at 340nm, the GST activity can be calculated.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvette (light path 1cm), low-temperature centrifuge, water bath, pipette, distilled water.