Product description
Glutamine amidotransferase (GLS, EC 3.5.1.2) is a type of amidase that catalyzes the hydrolysis of glutamine to glutamate and ammonia, playing a significant regulatory role in nitrogen metabolism, especially in regulating free ammonia levels and urea metabolism. GLS catalyzes the hydrolysis of glutamine to L-glutamate and ammonia. Ammonia, in a strong alkaline environment, reacts with hypochlorite and phenol to form the water-soluble dye indigo blue, which has a stable solution color. It has a characteristic absorption peak at 630nm, and by detecting the rate of increase of ammonia, the enzyme activity can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, pipette, mortar and pestle.