Product description
Fructose-6-phosphate (F6P) is an intermediate product of sugar metabolism and gluconeogenesis pathways, widely present in animals, plants, and microorganisms. This assay kit provides a simple, sensitive, and rapid determination method: Fructose-6-phosphate (F6P) under the successive action of phosphoglucose isomerase and glucose-6-phosphate dehydrogenase reduces NADP+ to NADPH. The F6P content in the sample can be calculated by detecting the increase of NADPH at 340nm.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvette (optical path 1cm), desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.