Fructose-1,6-bisphosphatase (FBP) Assay Kit (Visible Colorimetric Method) (GLUAF-K004M)

Cat.No.

GLUAF-K004M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Fructose-1,6-bisphosphatase, also known as fructose 1,6-bisphosphate aldolase (FBP, EC 3.1.3.11), is a key enzyme in the gluconeogenesis pathway. Different gluconeogenic substrates are converted to 1,6-bisphosphate fructose under the action of various enzymes and then hydrolyzed to fructose 6-phosphate and inorganic phosphate by FBP. The abnormal expression of this enzyme is closely related to certain diseases. This assay kit provides a simple, sensitive, and rapid method for determination: FBP catalyzes the reaction of 1,6-bisphosphate fructose and water to produce fructose 6-phosphate and inorganic phosphate, which interacts with an enzyme complex. The NADPH produced in this process then reacts with a specific color-developing probe to form a colored substance. By detecting the increase rate of this colored substance, the activity of FBP can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, temperature-controlled water bath, desktop centrifuge, pipette, mortar and pestle, and distilled water.