Product description
Fructose-1,6-bisphosphatase, also known as fructose 1,6-bisphosphate aldolase (FBP, EC 3.1.3.11), is a key enzyme in the gluconeogenesis pathway. Different gluconeogenic substrates are converted to 1,6-bisphosphate fructose under the action of various enzymes and then hydrolyzed to fructose 6-phosphate and inorganic phosphate by FBP. The abnormal expression of this enzyme is closely related to certain diseases. This assay kit provides a simple, sensitive, and rapid method for determination: FBP catalyzes the reaction of 1,6-bisphosphate fructose and water to produce fructose 6-phosphate and inorganic phosphate, which interacts with an enzyme complex. The NADPH produced in this process then reacts with a specific color-developing probe to form a colored substance. By detecting the increase rate of this colored substance, the activity of FBP can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), temperature-controlled water bath, desktop centrifuge, pipette, mortar and pestle, and distilled water.