Product description
Fructose-1,6-bisphosphatase, also known as fructose-1,6-bisphosphate phosphatase (FBP, EC 3.1.3.11), exists in two forms in photosynthetic cells. Cytosolic FBP is mainly present in the cytoplasm and is involved in sucrose synthesis and gluconeogenesis pathways; chloroplastic FBP is present in chloroplasts, where it plays a regulatory role in the carbon dioxide assimilation pathway. FBP catalyzes the conversion of fructose-1,6-bisphosphate and water to fructose-6-phosphate and inorganic phosphate, and then reacts with the enzyme complex, accompanied by the generation of NADPH. By detecting the increase rate of NADPH at 340 nm, the activity of FBP enzyme can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, constant temperature water bath, benchtop centrifuge, pipettes, mortar, and distilled water.