Product description
FFA is both a product of fat hydrolysis and a substrate for fat synthesis. The concentration of FFA is related to lipid metabolism, sugar metabolism, and endocrine function, and can also reflect quality changes during food storage. Under weakly acidic conditions, FFA reacts with copper salts to form copper soap, which has a characteristic absorption peak at 715nm. Within a certain range, the content of free fatty acids is linearly related to the degree of color development.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, water bath, pipette, mortar, ice.