Product description
Formate dehydrogenase (FDH, EC 1.2.1.2) belongs to the D-2-hydroxy acid dehydrogenase class and is widely used in the regeneration of coenzyme NADH. This kit utilizes FDH to catalyze the irreversible reaction of formate and NAD+ to produce carbon dioxide and NADH. By detecting the formation rate of NADH at 340nm, the activity of FDH can be calculated.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvettes (optical path 1cm), desktop centrifuge, pipettes, mortar and pestle, ice, and distilled water.