Ferric Chelate Reductase (FCR) Activity Assay Kit (OSRAF-K030M)

Cat.No.

OSRAF-K030M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Ferric chelate reductase (FCR) catalyzes the reduction of Fe3+ in ferric chelates to Fe2+, which plays an important role in the absorption of iron elements in some species. FCR can catalyze the reduction of Fe3+ to Fe2+, which then reacts with ferrozine to form a purple-red compound that has a characteristic absorption peak at 562nm. The enzyme activity can be determined by measuring the increase rate at 562nm.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, water bath or incubator, low-temperature centrifuge, distilled water