Product description
Exo-β-1,4-glucanase, also known as cellobiohydrolase (CBH) (EC 3.2.1.91), is found in bacteria, fungi, and animals, and is one of the components of the cellulase system. This enzyme acts on β-1,4-glycosidic bonds, cutting off one cellobiose (reducing sugar) molecule at a time. Under alkaline conditions, the produced reducing sugars react with 3,5-dinitrosalicylic acid to form a brown-red substance, which has the maximum absorption peak at 540nm, thus determining the enzyme activity of exo-β-1,4-glucanase.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, centrifuge, pipette, mortar, ice, and distilled water.