Product description
DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) is a stable free radical widely used to quantitatively determine the antioxidant capacity of biological samples and food. The method is based on the characteristic strong absorption of DPPH radicals with a single electron at 517nm, which gives their alcoholic solution a purple color. In the presence of a free radical scavenger, the absorption gradually disappears due to the pairing of the single electron, the lighter the color, the lower the A value, allowing for the quantitative analysis of the DPPH scavenging ability in the sample.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), centrifuge, pipette, mortar, ice, methanol, anhydrous ethanol, and distilled water.