Dihydroflavonol 4-Reductase (DFR) Assay Kit (PMSAD-K005S)

Cat.No.

PMSAD-K005S

Method

Spectrophotometry

Product size

24T

Product description

Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a key enzyme in the synthesis metabolism of anthocyanins, responsible for reducing three types of dihydroflavonols into colorless anthocyanidins. It plays an important role in determining the color of plant flowers, leaves, fruits, and other economic organs, as well as their nutritional quality. Dihydroflavonol 4-reductase (DFR) acts on dihydroquercetin to produce catechin, which can condense with vanillin to form a red compound, and has a characteristic absorption peak at 500nm to determine the enzyme activity of DFR.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), tabletop centrifuge, pipette, mortar, ethyl acetate, absolute ethanol, ice.