Product description
Debranching enzyme (DBE) (EC 3.2.1.68) belongs to the family of starch hydrolases and specifically hydrolyzes the α-1,6-glycosidic bonds of branched starch to produce linear glucose chains, playing an important role in adjusting the chain length of branched starch molecules. The 3,5-dinitrosalicylic acid method is used to determine the reducing sugars produced by DBE-catalyzed branched starch, and the enzyme activity of DBE is obtained by measuring the change in the content of reducing sugars.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), pipette, water bath, desktop centrifuge, mortar and pestle, ice, distilled water.