Product description
This kit provides a rapid and sensitive detection method: D-lactate is converted to pyruvate under the action of D-lactate dehydrogenase, reducing NAD+ to NADH; to ensure the smooth progress of this reaction, additional enzymes are added to further decompose pyruvate; the generated NADH reacts with a specific color-developing agent to produce a colored substance with a maximum absorption peak at 450nm. By detecting the increase at 450nm, the content of D-lactate can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), pipette, balance, mortar and pestle, centrifuge.