Product description
Creatine kinase (CK, EC 2.7.3.2) is primarily found in the heart, muscle, and brain tissues, and catalyzes the reversible phosphotransfer reaction between creatine and ATP, playing an important role in energy metabolism, muscle contraction, and ATP regeneration. CK catalyzes the reaction of ATP and creatine to form phosphocreatine, which is quickly hydrolyzed to phosphate, while ATP and the generated ADP remain stable; the enzyme activity is detected by measuring the amount of phosphate released from phosphocreatine using a phosphorus determination reagent.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.