Product description
Creatine is an endogenous compound that functions to maintain a high ATP/ADP ratio in the body through phosphorylation by creatine kinase. Creatine supplements are used as performance-enhancing agents and are also used to treat muscle and neurodegenerative diseases. Therefore, the detection of creatine has a wide range of applications in research and development. This assay kit utilizes creatine enzyme to specifically act on creatine to generate creatinine, which is then oxidized by creatinine oxidase to produce hydrogen peroxide. Hydrogen peroxide reacts with a color reagent to present a pink-red color, and the colored substance has a maximum absorption peak at 520nm, from which the creatine content can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), pipette, centrifuge, distilled water.