Product description
Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is a key enzyme in plant secondary metabolism, especially in lignin synthesis, closely related to plant growth and development and resistance to pathogen invasion. This assay kit provides a simple, sensitive, and rapid method for determination: CAD catalyzes the conversion of cinnamyl alcohol and NADP+ into cinnamaldehyde and NADPH, which then reacts with a specific color developer to produce colored substances. By detecting the increase rate of colored substances, the CAD enzyme activity can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), tabletop centrifuge, pipette, mortar, ice, and distilled water.