Product description
Chymotrypsin (EC 3.4.21.1), also known as trypsin, is a proteolytic enzyme secreted by the pancreas, acting as an endopeptidase that specifically hydrolyzes the carboxyl-terminal peptide bonds of tyrosine and phenylalanine in protein chains. Clinically, chymotrypsin is used for liquefaction of sputum, effective for both purulent and non-purulent sputum, and also for wound healing after trauma or surgery. This assay kit uses chymotrypsin to catalyze the hydrolysis of N-succinyl-Ala-Ala-Ala-Phe-p-nitroanilide (SAAPFpNA) to produce p-nitroaniline, which has a maximum absorption peak at 405nm. The enzyme activity of chymotrypsin can be determined by measuring the rate of increase in absorbance.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), desktop centrifuge, pipette, balance, mortar and pestle, ice, and distilled water.