Product description
Various microorganisms, animals, and plants can produce chondroitinases. Higher plants do not contain chondroitin, which is one of the components of fungal cell walls, but when plants are infected by pathogens, the activity of chondroitinase increases rapidly. Therefore, this enzyme is related to the plant's resistance to pathogens and is an important disease-related protein. Chondroitinases mainly hydrolyze β-1,4-glycosidic bonds in chondroitin polymers. Under the action of snail enzyme, they are completely hydrolyzed into N-acetylglucosamine monomers, which further react with potassium ferricyanide and are detected at 420nm to calculate the chondroitinase activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, water bath, low-temperature centrifuge, hydrochloric acid, distilled water.