Product description
Various microorganisms, animals, and plants can produce chitinases. Higher plants do not contain chitin, which is one of the components of fungal cell walls, but when plants are infected by pathogens, the activity of chitinase increases rapidly. Therefore, this enzyme is related to the plant's resistance to pathogens and is an important disease-related protein. Chitinases mainly hydrolyze β-1,4-glycosidic bonds in chitin polymers. Based on the position of hydrolysis, they can be divided into endo-chitinases and exo-chitinases. Exo-chitinases act on chitin to produce N-acetylglucosamine monomers, which further react with potassium ferricyanide and are detected at 420nm to calculate the exo-chitinase activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, water bath, low-temperature centrifuge, hydrochloric acid, distilled water.