Product description
Cellulose is one of the main components of the plant cell wall. The amount of cellulose is related to the development of plant cell tissue and thus affects the crop's ability to resist lodging and diseases. Cellulose is a polysaccharide composed of glucose units, which can be hydrolyzed into glucose under acidic conditions. Then, under the action of concentrated sulfuric acid, monosaccharides are dehydrated to form furfural compounds. Anthrone reagent reacts with furfural compounds to form a blue-green substance. The blue-green substance has a maximum absorption peak at 620nm upon spectral scanning, and the cellulose content is obtained.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, pipettes, 80% ethanol, acetone, concentrated sulfuric acid, mortar, and distilled water.