Product description
During normal life processes, reactive oxygen species (ROS) are essential for maintaining life and are in a dynamic balance of continuous production and clearance, always maintaining a normal level, which can be considered an effective defense system of the body. The physiological functions of ROS include participation in electron transfer, bactericidal action, and substance metabolism within the body. The kit uses the chemical fluorescent indicator, 2’,7’-Dichlorofluorescin Diacetate (DCFH-DA), to detect reactive oxygen species in cells. DCFH can be oxidized by various reactive oxygen species into a substance that emits a fluorescent signal, namely 2’,7’-Dichlorofluorescein (DCF). The obtained DCF fluorescence signal is not produced by the oxidation of a single reactive oxygen species, so the reactive oxygen species measured with this fluorescent indicator reflects the total oxidative capacity of all reactive oxygen species. This fluorescence signal has a maximum peak at an excitation wavelength of 488nm and an emission wavelength of 525nm, and its fluorescence intensity is proportional to the level of reactive oxygen species.
Additional Materials and Equipments Required
Fluorescence microplate reader, low-temperature desktop centrifuge, pipette, black 96-well plate