Product description
Caspase-3, also known as CPP32, Yama, or apopain, belongs to the CED-3 subfamily and is a key enzyme in the process of apoptosis. Caspase-3 breaks down the substrate Ac-DEVD-pNA to produce yellow p-nitroaniline (pNA), which has a maximum absorption peak at 405nm. The enzyme activity of Caspase-3 can be determined by measuring the rate of increase in absorbance.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), desktop centrifuge, pipette, balance, mortar and pestle, ice, and distilled water.