Product description
Calcium is a metallic element found in the form of calcium carbonate in animal bones, clam shells, and eggshell. This kit uses the ability of calcium ions in alkaline solution to combine with o-cresolphthalein complexone (OCPC) to form a red complex, with the addition of a magnesium ion chelator to eliminate magnesium ion background interference. The absorbance of the colored complex at 575nm is measured to calculate the total calcium content.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, centrifuge, calcium-free distilled water.