Product description
Ca2+ is an important signaling molecule within the cell, and the concentration of Ca2+ in the cytoplasm is maintained at a low level, primarily by the action of Ca2+-ATPase, which catalyzes the hydrolysis of ATP to produce ADP and inorganic phosphate. The enzyme activity is determined by measuring the amount of inorganic phosphate.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.