Product description
Common methods for determining amylose include potentiometric determination, polarimetric analysis, or colorimetric methods based on the binding force of iodine with amylose. However, these methods have uncertainties because amylopectin-iodine complexes also form during this process, leading to overestimation of amylose content, which requires correction. This kit utilizes the characteristic of kidney bean globulin A, which only binds with amylopectin and not with amylose, to separate them. Then, an enzyme complex that hydrolyzes starch hydrolyzes starch into glucose, and the content of straight chain, branched chain, and total starch is determined by detecting the glucose content.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), water bath, pipettes, mortar and pestle, dimethyl sulfoxide (DMSO), ethanol, acetic acid.