Product description
Branched-chain amino acid transaminase (BCAT, E.C.2.6.1.42) belongs to the class of transaminases that use pyridoxal phosphate as a coenzyme. This enzyme is widely distributed and has been found in prokaryotes and most eukaryotes. BCAT catalyzes the specific transfer of amino groups from L-type amino acids to α-ketoglutarate, forming the corresponding branched-chain α-keto acids and glutamate; then, a specific enzyme complex acting on glutamate decomposes glutamate and reacts with a color developer to form a yellow substance, which has a maximum absorption peak at 450nm, allowing for the determination of BCAT enzyme activity. The enzyme-catalyzed reaction is: L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, water bath, pipettes, mortar and pestle, ice, and distilled water.