Product description
Bound starch synthase GBSS (EC 2.4.1.21) exists in a bound state within the amyloplast and catalyzes the elongation of starch chains, primarily responsible for the synthesis of amylose. GBSS catalyzes the reaction between ADPG and starch primer (glucan), transferring glucose molecules to the starch primer while generating ADP. An enzymatic mixture added to the reaction system sequentially catalyzes the reduction of NADP+ to NADPH, and the amount of NADPH generated is proportional to the amount of ADP generated in the previous step. The traditional method involves detecting the increase in NADPH at 340nm, but this method has low sensitivity and is prone to interference from pigments (such as green leaves). This kit provides a simple, sensitive, and rapid determination method: the NADPH produced in this enzymatic process reacts with a specific colorimetric probe to form a colored substance, and the increase in this colored substance at 450nm is detected to calculate the GBSS enzyme activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.