Product description
Betaine is a biological alkaloid widely distributed in plants, animals, and microorganisms. Under strong acidic conditions, it reacts with Reinhardt's salt to produce a red precipitate, which dissolves in acetone to form a pink solution with a characteristic absorption peak at 525nm. By measuring the absorbance at 525nm, the content of betaine in the sample can be calculated.
Additional Materials and Equipments Required
Spectrophotometer, 1mL glass cuvette (light path 1cm), pipette, balance, centrifuge, methanol, petroleum ether, hydrochloric acid, acetone, and distilled water.