Product description
β-Xylanase (EC3.2.1.37) is an important class of xylan degradation hydrolases that exist in plants, bacteria, and fungi. It primarily catalyzes the cleavage of xylobiose and xylooligosaccharides from the non-reducing end into xylose monomers, which can be used as a carbon source for microbial fermentation. Additionally, β-xylanase can be used as a biological bleaching agent in the paper industry, which is more environmentally friendly than traditional bleaching methods and has a wide range of application values. β-Xylanase catalyzes the production of p-nitrophenol (PNP) from p-nitrophenyl-β-D-xyloside, which has a characteristic absorption peak at 405nm. The enzyme activity can be calculated by measuring the increase in absorbance at 405nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, low-temperature centrifuge, balance, mortar and pestle, ice, and distilled water.