Product description
β-Xylanase (EC3.2.1.37) is an important class of xylan degradation hydrolases that exist in plants, bacteria, and fungi. It primarily catalyzes the cleavage of xylobiose and xylooligosaccharides from the non-reducing end into xylose monomers, which can be used as a carbon source for microbial fermentation. Additionally, β-xylanase can be used as a biological bleaching agent in the paper industry, which is more environmentally friendly than traditional bleaching methods and has a wide range of application values. β-Xylanase catalyzes the production of p-nitrophenol (PNP) from p-nitrophenyl-β-D-xyloside, which has a characteristic absorption peak at 405nm. The enzyme activity can be calculated by measuring the increase in absorbance at 405nm.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (path length 1cm), pipettes, low-temperature centrifuge, balance, mortar, ice, and distilled water.