Product description
β-Glucuronidase (β-GUS, EC 3.2.1.31) is a widely distributed hydrolase enzyme found in animals and microorganisms; however, it is not present in most plant cells and many bacteria and fungi, making the GUS gene widely used as a reporter gene in transgenic plants, bacteria, and fungi. This method uses 4-methylumbelliferyl β-D-glucuronide (4-MUG) as a substrate, which is hydrolyzed by GUS to produce 4-methylumbelliferone (4-MU) and β-D-glucuronide. The hydroxyl group of 4-MU is ionized and excited by 365nm light, emitting fluorescence at 455nm, which is used to calculate GUS enzyme activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.