Product description
β-Glucosidase (β-GC, EC 3.2.1.21) is widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the hydrolysis of β-D-glucoside bonds and is related to the relaxation and strengthening of the plant cell wall during plant cell growth and development. It is also associated with plant cell recognition and the production of some signaling molecules. β-Glucosidase (β-GC) can hydrolyze p-nitrophenyl-β-D-glucopyranoside to produce p-nitrophenol (PNP), which has a maximum absorption peak at 405nm. The enzyme activity can be calculated by measuring the rate of increase in absorbance at 405nm.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (path length 1cm), benchtop centrifuge, water bath, pipettes, mortar, ice.