Product description
β-Glucosidase (β-GC, EC 3.2.1.21) is widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the hydrolysis of β-D-glucoside bonds and is related to the relaxation and reinforcement of plant cell walls during growth and development, as well as to plant cell recognition and the production of some signaling molecules. β-Glucosidase (β-GC) can hydrolyze p-nitrophenyl-β-D-glucopyranoside to produce p-nitrophenol (PNP), which has a maximum absorption peak at 405nm. The increase in absorbance rate is used to calculate β-glucosidase activity.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), desktop centrifuge, water bath, pipette, mortar and pestle, ice.