Product description
Asparaginase (EC 3.5.1.1, ASNase) is an amide hydrolase that can remove the amino group from L-asparagine to produce L-aspartic acid and ammonia. This enzyme has anti-tumor activity and is widely used in food and medicine. ASNase catalyzes the hydrolysis of asparagine into aspartic acid and ammonia. Ammonia, in a strong alkaline environment, reacts with hypochlorite and phenol to form the water-soluble dye indigo blue, which has a stable solution color. It has a characteristic absorption peak at 630nm, and by detecting the rate of increase of ammonia, the enzyme activity can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, pipette, mortar and pestle.