Product description
Alcohol dehydrogenase (ADH, EC 1.1.1.1) is found in many organisms, capable of breaking down toxic alcohols in humans and many other animals; in yeast and many bacteria, some alcohol dehydrogenases catalyze the reverse reaction as part of fermentation. This kit provides a rapid and sensitive detection method: ADH catalyzes the reaction of ethanol and NAD+ to produce acetaldehyde and NADH. The produced NADH reacts with a specific color-developing agent to form a yellow substance with a maximum absorption peak at 450nm. By detecting the increase rate of this yellow substance at 450nm, the activity of ADH can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.