Product description
Albumin carries a positive charge in a buffer solution at pH 4.2 and can combine with the negatively charged dye bromocresol green to form a blue-green complex in the presence of a non-ionic surfactant. The complex has an absorption peak at 630nm, and the color intensity is directly proportional to the concentration of albumin.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), pipette, centrifuge, distilled water.