Alanopine Dehydrogenase (ADH) Activity Assay Kit (GLYAA-K006M)

Cat.No.

GLYAA-K006M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Marine invertebrates primarily have four anaerobic metabolic pathways, among which the glucose-opine pathway plays an important role in the early stages of anaerobic metabolism. The unique Opine Dehydrogenases (OpDHs) in invertebrates ensure the smooth progress of this process. Alanopine Dehydrogenase (ADH; EC 1.5.1.17) is one of the Opine Dehydrogenases (OpDHs) series enzymes. ADH catalyzes the reaction between pyruvate and the specific substrate alanine to form the corresponding amino acid, while oxidizing NADH. By measuring the decrease in NADH at the characteristic absorption wavelength of 340nm, the activity of ADH can be determined.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.