Product description
Aconitase (EC 4.2.1.3) is an enzyme in the citric acid cycle that catalyzes the conversion of citrate to isocitrate. Citrate itself is not easily oxidized. Under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon atom to the α-carbon atom, forming isocitrate that is easily dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions. Aconitase catalyzes the conversion of citrate to isocitrate, which is then oxidized and decarboxylated by isocitrate dehydrogenase to reduce NADP+ to NADPH. By measuring the increase in light absorption at 340nm, the activity of aconitase can be determined.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, low-temperature centrifuge, mortar and pestle.