Product description
Proteases are widely present in animal viscera, plant stems, leaves, fruits, and microorganisms. Acidic protease (ACPT) hydrolyzes casein to produce tyrosine under acidic conditions; tyrosine reacts with Folin-Ciocalteu reagent to form a blue compound; this blue substance has a characteristic absorption peak at 680nm, allowing for the determination of acidic protease activity. Since the substrate casein itself contains various amino acids, a control with casein must be set during the detection process to subtract interfering background values and exclude false positives.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), water bath, magnetic stirrer, pipette gun.