Product description
Acetyl CoA is an important intermediate metabolic product in energy substance metabolism, acting as a hub in the metabolism of sugars, fats, and proteins. These three major nutrients converge into a common metabolic pathway-the citric acid cycle and oxidative phosphorylation-to be completely oxidized into carbon dioxide and water, releasing energy for ATP synthesis. It is also a precursor for the synthesis of physiologically active substances such as fatty acids, ketone bodies, cholesterol, and their derivatives. Malate dehydrogenase can catalyze the reaction of malate and NAD to produce oxaloacetate and NADH. Citrate synthase can catalyze the reaction of acetyl CoA and oxaloacetate to produce citrate and CoA. Using the coupled reaction of malate dehydrogenase and citrate synthase, the content of acetyl CoA is proportional to the generation of NADH, and the increase in absorbance at 340nm reflects the amount of acetyl CoA.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.