Product description
Acetic acid is widely present in food, beverages, and other materials, making it an important indicator for detection. Acetic acid is converted into acetyl-CoA and ADP from acetic acid and ATP under the action of acetate kinase and phosphotransacetylase, followed by the catalytic oxidation of NADH to NAD+ by pyruvate kinase and lactate dehydrogenase. The decrease in NADH at 340nm is measured to calculate the amount of acetic acid.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.