Product description
α-Xylanase (EC 3.2.1.177) is a type of xylan degradation hydrolase enzyme found in plants, bacteria, and fungi, promoting the hydrolysis of non-reducing terminal α-D-xylose residues, releasing α-D-xylose. α-Xylanase catalyzes the production of p-nitrophenol (PNP) from p-nitrophenyl-α-D-xyloside, which has a characteristic absorption peak at 405nm. The enzyme activity can be calculated by measuring the increase in absorbance at 405nm.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (path length 1cm), pipettes, low-temperature centrifuge, balance, mortar, ice, and distilled water.