Product description
6-Phosphogluconate dehydrogenase (6-PGDH, EC 1.1.1.43) is one of the key enzymes in the pentose phosphate pathway, playing an important role in maintaining the level of NADPH in cells, which is closely related to the energy balance, growth rate, and cell vitality of the organism. This assay kit provides a simple, sensitive, and rapid method for determination: 6-PGDH catalyzes the reaction of 6-phosphogluconate and NADP+ to produce NADPH. Subsequently, it reacts with a specific color-developing probe to form a colored substance. By detecting the increase rate of this colored substance, the activity of 6-PGDH can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature centrifuge, water bath, pipette, and distilled water.