Product description
4-Coumarate:CoA ligase (4CL, EC 6.2.1.12) is one of the key enzymes in lignin biosynthesis, located at the turning point between the phenylpropanoid pathway and the lignin-specific synthesis pathway, mainly catalyzing the formation of cinnamic acid into the corresponding cinnamic acid CoA esters. This enzyme is mainly found in higher plants, yeast, and fungi. Studying this enzyme can explore the metabolic mechanisms of lignin deposition during the development of various biological cells, providing a basis for reducing the stone cell content in fruits to improve their quality. 4CL catalyzes 4-coumaric acid and CoA to form 4-coumaric acid CoA, and the generation rate of 4-coumaric acid CoA at 333nm can reflect the activity of 4CL.
Additional Materials and Equipments Required
Microplate reader, 96-well plate (UV plate), low-temperature tabletop centrifuge, pipette, mortar, ice, and distilled water.