Product description
γ-Glutamyl transpeptidase (γ-GT/GGT) in serum mainly comes from the liver and bile system. Therefore, its activity significantly increases when there are diseases or injuries in the liver and gall system, such as obstructive jaundice, biliary cirrhosis, cholangitis, and cholecystitis; alcohol consumption and medication can also cause an increase in γ-glutamyl transpeptidase activity. Thus, the determination of γ-glutamyl transpeptidase is of great clinical significance for the detection of liver and biliary diseases. L-γ-glutamyl-3-carboxy-4-phenylazoaniline is used as a substrate, and diglycine serves as the acceptor of the glutamyl group. Under the action of GGT, a colored product, 5-amino-2-nitrobenzoic acid, is formed, which has a characteristic absorption peak at 405nm. The activity of γ-glutamyl transpeptidase in the sample can be calculated by measuring the rate of formation of this substance.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, centrifuge, pipette, distilled water.