Co-Immunoprecipitation (Co-IP) Kit (Plant) (HZKIT006P)

Co-Immunoprecipitation (Co-IP) is an investigative technique designed to explore the interactions between proteins. It relies on the specific binding of an antibody to its antigen as a means to precipitate the protein complex. During the process, the antibody recognizes and binds to its target protein within a cell lysate. Subsequently, the mixture is incubated with Protein A/G conjugated to Sepharose beads or magnetic beads. The complex is then isolated through centrifugation or by using a magnetic field. The resulting Protein A/G bead-antibody-target protein complexes are eluted, and the antigen-antibody bond is disrupted by the application of heat and a reducing agent. The supernatant, containing the antibody, target protein, and a small amount of non-specific proteins, is collected for further analysis. This is typically done using Western Blotting (WB) or mass spectrometry (MS) to identify and characterize the proteins involved in the interaction.. In contrast to the Co-IP Kit for animal, this kit covers special plant lysis buffer and making it ideally suited for the study of plant protein interactions.